Anti-Human IL1B/IL1F2 Antibody (SAA0368) (HF943107)

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Overview

Catalog No.	HF943107
Species reactivity	Human
Applications	ELISA, FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	SAA0368
Conjugation	Unconjugated
Clonality	Monoclonal
Target	Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta,IL-1β
Concentration	1 mg/ml
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01584
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti-human IL1B antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1B antibody monoclonal antibody (Catalog # HF943107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IL1B antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1B antibody monoclonal antibody (Catalog # HF943107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet