Catalog No. | HM814107 |
---|---|
Applications | FCM |
Host species | Mouse |
Isotype | IgG1, kappa |
Clone ID | H4H2 |
Clonality | Monoclonal |
Target | HLA-B, HLA class I histocompatibility antigen, B alpha chain, Human leukocyte antigen B, HLAB, HLA-B*27 |
Endotoxin level | Please contact with the lab for this information. |
Purity | >95% as determined by SDS-PAGE. |
Purification | Protein A/G purified from cell culture supernatant. |
Accession | P01889 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
Note | For research use only. |
Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # HM814107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # HM814107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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