Anti-Mouse LY6E/SCA-2 Antibody (SAA1998) (MS714107)

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Datasheet

Overview

Catalog No.

MS714107

Description

Anti-Mouse LY6E/SCA-2 Antibody (SAA1998) [SAA1998] (MS714107) is a mouse monoclonal antibody detecting SCA-2 in FCM, WB. Suitable for Human and Mouse.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human, Mouse

Applications

FCM, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1998

Clonality

Monoclonal

Target

SCA-2, TSA-1, 9804, Retinoic acid-induced gene E protein, TSA1, Lymphocyte antigen 6E, Ly-6E, Stem cell antigen 2, Thymic shared antigen 1, SCA2, RIGE, LY6E, RIG-E

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q64253

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Lymphocyte antigen 6E (LY6E/SCA-2) is a ~14 kDa protein. GPI-anchored cell surface protein that regulates T-lymphocytes proliferation, differentiation, and activation. Regulates the T-cell receptor (TCR) signaling by interacting with component CD3Z/CD247 at the plasma membrane, leading to CD3Z/CD247 phosphorylation modulation. Restricts the entry of murine coronavirus, mouse hepatitis virus, by interfering with spike protein-mediated membrane fusion. Also plays an essential role in placenta formation by acting as the main receptor for syncytin-A (SynA). Therefore, participates in the normal fusion of syncytiotrophoblast layer I (SynT-I) and in the proper morphogenesis of both fetal and maternal vasculatures within the placenta.

1. Noda, S. et al. (1996) The Journal of experimental medicine 183, 2355-60. PMID: 8642345

2. Kosugi, A. et al. (1998) The Journal of biological chemistry 273, 12301-6. PMID: 9575182

3. Pfaender, S. et al. (2020) Nature microbiology 5, 1330-1339. PMID: 32704094

4. Bacquin, A. et al. (2017) Journal of virology 91. PMID: 28679758

5. Langford, MB. et al. (2018) Scientific reports 8, 3961. PMID: 29500366

6. Wu, M. et al. (2015) The Journal of biological chemistry 290, 24509-18. PMID: 26276394

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-LY6E/SCA-2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human LY6E/SCA-2 (Catalog No: HS714011), 50ng
Lane 2: recombinant human LY6E/SCA-2 (Catalog No: HS714011), 30ng
Lane 3: recombinant human LY6E/SCA-2 (Catalog No: HS714011), 20ng
Lane 4: recombinant human LY6E/SCA-2 (Catalog No: HS714011), 10ng

References

Datasheet

Anti-Mouse LY6E/SCA-2 Antibody (SAA1998).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse LY6E/SCA-2 Antibody (SAA1998) (MS714107)

Overview

Images

References

Datasheet

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