Anti-Human CD8A Antibody (mab1) (HF815107)

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Overview

Catalog No.	HF815107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Conjugation	Unconjugated
Clonality	Monoclonal
Target	T-cell surface glycoprotein CD8 alpha chain, CD8a, T-lymphocyte differentiation antigen T8/Leu-2, MAL, CD8A
Concentration	0.61 mg/ml
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01732
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti- human CD8 antibody.
Human RPMI-8226 cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD8 antibody monoclonal antibody (Catalog # HF815107 , Red Histogram) at a concentration of 20ug/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD8A Antibody (mab1).

References

Datasheet