Anti-Human CD85d/LILRB2 Antibody (J19.H1) (HV255107)

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Overview

Catalog No.	HV255107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	J19.H1
Clonality	Monoclonal
Target	CD85 antigen-like family member D, Monocyte/macrophage immunoglobulin-like receptor 10, MIR-10, ILT4, LIR-2, ILT-4, Immunoglobulin-like transcript 4, MIR10, CD85d, Leukocyte immunoglobulin-like receptor subfamily B member 2, Leukocyte immunoglobulin-like receptor 2, LIR2, LILRB2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q8N423
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

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Bioactivity

Detects CD85d/LILRB2/ILT4 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD85d antibody.CD85d Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD85d antibody monoclonal antibody (Catalog # HV255107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD85d antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD85d antibody monoclonal antibody (Catalog # HV255107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet