Anti-Human CD54/ICAM1 Antibody (SAA0025) (HY321107)

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Datasheet

Overview

Catalog No.	HY321107
Description	Anti-Human CD54/ICAM1 Antibody (SAA0025) [SAA0025] (HY321107) is a human monoclonal antibody detecting ICAM1 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Human
Isotype	IgG1, lambda
Clone ID	SAA0025
Clonality	Monoclonal
Target	ICAM1, Major group rhinovirus receptor, CD54, ICAM-1, Intercellular adhesion molecule 1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P05362
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Intercellular adhesion molecule 1 (CD54/ICAM1) is a ~57 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM:ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1. 1. Dustin, ML. et al. (1986) Journal of immunology (Baltimore, Md. : 1950) 137, 245-54. PMID: 3086451 2. Simmons, D. et al. (1988) Nature 331, 624-7. PMID: 3340213 3. Lacouture, C. et al. (2024) Nature communications 15, 407. PMID: 38195629 4. Diamond, MS. et al. (1990) The Journal of cell biology 111, 3129-39. PMID: 1980124 5. van Buul, JD. et al. (2007) The Journal of cell biology 178, 1279-93. PMID: 17875742 6. Hayashi, T. et al. (2001) Digestion 63 Suppl 1, 87-92. PMID: 11173916
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti- human CD54 antibody.
Human RPMI-8226 cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD54 antibody monoclonal antibody (Catalog # HY321107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD54/ICAM1 Antibody (ABS1990).