Catalog No. | HT495107 |
---|---|
Species reactivity | Human |
Applications | FCM |
Host species | Human |
Isotype | IgG1 |
Clone ID | SAA0050 |
Conjugation | Unconjugated |
Clonality | Monoclonal |
Target | PROML1, PROM1, Prominin-1, Antigen AC133, CD133, Prominin-like protein 1 |
Concentration | 1 mg/ml |
Endotoxin level | Please contact with the lab for this information. |
Purity | >95% as determined by SDS-PAGE. |
Purification | Protein A/G purified from cell culture supernatant. |
Accession | O43490 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
Note | For research use only. |
Flow-cytometry using anti- human CD133 antibody.
Human CACO-2 cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD133 antibody monoclonal antibody (Catalog # HT495107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414 ) and cells analysed on a NovoCyte Flow Cytometer.
SEC-HPLC detection for Anti-Human CD133/PROM1 Antibody (ABS1140).
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