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Anti-Human CD100/SEMA4D Antibody (VX15/2503) (HV563107)

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  • Datasheet
Overview
Catalog No.HV563107
Species reactivityHuman
ApplicationsFCM
Host speciesHuman
IsotypeIgG4, kappa
Clone IDVX15/2503
Clonality Monoclonal
Target A8, Semaphorin-4D, GR3, BB18, SEMAJ, C9orf164, CD100, SEMA4D
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q92854
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Bioactivity

    SEC-HPLC detection for Anti-Human CD100/SEMA4D Antibody (VX15/2503).

  • Flow Cytometry

    Flow-cytometry using anti-human CD100 antibody.CD100 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD100 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD100 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Bioactivity

    Detects recombinant human CD100/SEMA4D (Catalog No: HV563011) in indirect ELISA.

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