Anti-Human CD100/SEMA4D Antibody (VX15/2503) (HV563107)

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Overview

Catalog No.	HV563107
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG4, kappa
Clone ID	VX15/2503
Clonality	Monoclonal
Target	A8, Semaphorin-4D, GR3, BB18, SEMAJ, C9orf164, CD100, SEMA4D
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q92854
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human CD100/SEMA4D Antibody (VX15/2503).
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.CD100 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD100 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD100 antibody monoclonal antibody (Catalog # HV563107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet