Anti-Human KRAS Nanobody (SBT-100) (HF904023)

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50ug
100ug
1mg

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Overview

Catalog No.

HF904023

Description

Anti-Human KRAS Nanobody (SBT-100) [SBT-100] (HF904023) is a alpaca monoclonal antibody detecting KRAS in ELISA, IF. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IF

Host species

Alpaca

Isotype

VHH-His

Clone ID

SBT-100

Clonality

Monoclonal

Target

EC:3.6.5.2, GTPase KRas, GTPase KRas, N-terminally processed, K-Ras 2, KRAS, KRAS2, Ki-Ras, RASK2, c-K-ras, c-Ki-ras

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P01116

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

GTPase KRas is a ~21 kDa protein. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.

1. Gremer, L. et al. (2011) Human mutation 32, 33-43. PMID: 20949621

2. Bernal Astrain, G. et al. (2025) Nature communications 16, 647. PMID: 39809765

3. Yang, MH. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 10843-8. PMID: 22711838

4. Zimmermann, G. et al. (2013) Nature 497, 638-42. PMID: 23698361

5. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306

Note

For research use only

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Datasheet