Anti-Human CD47/MER6 Antibody (5F9-G4) (HC359023)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

HC359023

Description

Anti-Human CD47/MER6 Antibody (5F9-G4) [5F9-G4] (HC359023) is a human monoclonal antibody detecting Protein MER6 in ELISA, FACS, Functional assay. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, FACS, Functional assay

Host species

Human

Isotype

IgG1, kappa

Clone ID

5F9-G4

Clonality

Monoclonal

Target

Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q08722

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Leukocyte surface antigen CD47 (MER6) is a ~35 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical).

1. Latour, S. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 2547-54. PMID: 11509594

2. Piccio, L. et al. (2005) Blood 105, 2421-7. PMID: 15383453

3. Isenberg, JS. et al. (2009) The Journal of biological chemistry 284, 1116-25. PMID: 19004835

4. Lindberg, FP. et al. (1993) The Journal of cell biology 123, 485-96. PMID: 7691831

5. Gao, AG. et al. (1996) The Journal of biological chemistry 271, 21-4. PMID: 8550562

6. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621

7. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764

Note

For research use only

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