Anti-Human CD152/CTLA4 Antibody (SAA2550) (HB651043)

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100ug
1mg

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Datasheet

Overview

Catalog No.

HB651043

Description

Anti-Human CD152/CTLA4 Antibody (SAA2550) [SAA2550] (HB651043) is a rabbit monoclonal antibody detecting CD152 in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Isotype

scFv-His

Clone ID

SAA2550

Clonality

Monoclonal

Target

CD152, CTLA-4, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P16410

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cytotoxic T-lymphocyte protein 4 (CD152/CTLA4) is a ~24 kDa protein. Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. CD152 is the therapeutic target of ipilimumab (Yervoy).

1. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501

2. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502

3. Teft, WA. et al. (2006) Annual review of immunology 24, 65-97. PMID: 16551244

4. Linsley, PS. et al. (1991) The Journal of experimental medicine 174, 561-9. PMID: 1714933

5. Zheng, Y. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 1683-91. PMID: 18641304

6. Ramagopal, UA. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E4223-E4232. PMID: 28484017

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Human CD152/CTLA4 Antibody (SAA2550)
Western blot

Western blot analysis was performed using anti-SCFV-His polyclonal antibody at 1ug/mL on various samples.
Lane 1: Recombinant protein 100ng

References

Datasheet

Anti-Human CD152/CTLA4 Antibody (SAA2550).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD152/CTLA4 Antibody (SAA2550) (HB651043)

Overview

Images

References

Datasheet

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