Anti-Human CD49d/ITGA4 Antibody (SAA2539) (HY596023)

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Datasheet

Overview

Catalog No.

HY596023

Description

Anti-Human CD49d/ITGA4 Antibody (SAA2539) [SAA2539] (HY596023) is a rabbit monoclonal antibody detecting CD49D in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Isotype

scFv-His

Clone ID

SAA2539

Clonality

Monoclonal

Target

CD49 antigen-like family member D, CD49D, CD49d, ITGA4, Integrin alpha-4, Integrin alpha-IV, VLA-4 subunit alpha

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P13612

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Integrin alpha-4 (CD49d/ITGA4) is a ~114 kDa protein. Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1.

1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415

2. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536

3. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877

4. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072

Note

For research use only

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SDS-PAGE

SDS-PAGE for Anti-Human CD49d/ITGA4 Antibody (SAA2539)

References

Datasheet

Anti-Human CD49d/ITGA4 Antibody (SAA2539).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD49d/ITGA4 Antibody (SAA2539) (HY596023)

Overview

Images

References

Datasheet

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