Anti-HPV18 E7/Protein E7 Polyclonal Antibody (VK556024)

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK556024

Description

Anti-HPV18 E7/Protein E7 Polyclonal Antibody (VK556024) is a rabbit polyclonal antibody detecting Protein E7 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human papillomavirus type 18

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HPV18 E7/Protein E7 (Met1-Gln105).

Target

Protein E7, E7

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P06788

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein E7 is a ~11 kDa protein. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation.

1. Lau, L. et al. (2015) Science (New York, N.Y.) 350, 568-71. PMID: 26405230

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-E7 protein polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HPV18 E7 (Catalog No: VK556062)

References

Datasheet