Anti-Rotavirus A Outer capsid protein VP4 Polyclonal Antibody (VK676014)

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Datasheet

Overview

Catalog No.

VK676014

Description

Anti-Rotavirus A Outer capsid protein VP4 Polyclonal Antibody (VK676014) is a rabbit polyclonal antibody detecting VP4 capsid protein in ELISA, IHC, WB. Suitable for Human rotavirus A.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human rotavirus A

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Rotavirus A Outer capsid protein VP4 (Leu65-Leu223).

Target

VP4 capsid protein, VP4, Hemagglutinin

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P30214, P21284, P11196

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Outer capsid protein VP4 is a ~87 kDa protein. Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. It is subsequently lost, together with VP7, following virus entry into the host cell. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.

Note

For research use only

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References

Datasheet

Anti-Rotavirus A Outer capsid protein VP4 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Rotavirus A Outer capsid protein VP4 Polyclonal Antibody (VK676014)

Overview

Images

References

Datasheet

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