Anti-Human MTOR Antibody (SAA2296) (HW733013)

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Spec:

50ug
100ug
1mg

Number:

Overview
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Datasheet

Overview

Catalog No.

HW733013

Description

Anti-Human MTOR Antibody (SAA2296) [SAA2296] (HW733013) is a human monoclonal antibody detecting mTOR in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Human

Isotype

IgG1, kappa

Clone ID

SAA2296

Clonality

Monoclonal

Target

Serine/threonine-protein kinase mTOR, EC:2.7.11.1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Tyrosine-protein kinase mTOR, EC:2.7.10.2, MTOR

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P42345

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase mTOR is a ~288 kDa protein. Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). In response to nutrients, growth factors or amino acids, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E).

1. Park, IH. et al. (2002) The Journal of biological chemistry 277, 31423-9. PMID: 12087098

2. Kim, DH. et al. (2002) Cell 110, 163-75. PMID: 12150925

3. Hara, K. et al. (2002) Cell 110, 177-89. PMID: 12150926

4. Choi, JH. et al. (2002) EMBO reports 3, 988-94. PMID: 12231510

5. Kim, DH. et al. (2003) Molecular cell 11, 895-904. PMID: 12718876

6. Inoki, K. et al. (2003) Cell 115, 577-90. PMID: 14651849

7. Sarbassov, DD. et al. (2004) Current biology : CB 14, 1296-302. PMID: 15268862

8. Jacinto, E. et al. (2004) Nature cell biology 6, 1122-8. PMID: 15467718

9. Brugarolas, J. et al. (2004) Genes & development 18, 2893-904. PMID: 15545625

10. Sarbassov, DD. et al. (2005) Science (New York, N.Y.) 307, 1098-101. PMID: 15718470

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Human MTOR Antibody (SAA2296)

References

Datasheet

Anti-Human MTOR Antibody (SAA2296).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MTOR Antibody (SAA2296) (HW733013)

Overview

Images

References

Datasheet

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