Anti-ARHGAP21 Polyclonal Antibody (HA562014)

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Overview

Catalog No.

HA562014

Description

Anti-ARHGAP21 Polyclonal Antibody (HA562014) is a rabbit polyclonal antibody detecting ARHGAP21 in WB, IHC, ELISA. Suitable for Human, Mouse, Rat, Dog, and Cat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Dog, Cat, Bovine, Pig, African clawed frog, Alpaca, Camel, Ferret, Horse, Lion, Rabbit, Red fox, Sheep

Applications

WB,IHC,ELISA

Host species

Rabbit

Isotype

IgG

Species

Homo sapiens (Human)

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human ARHGAP21 (Gly1143-Ala1344).

Target

Rho GTPase-activating protein 21, Rho GTPase-activating protein 10, Rho-type GTPase-activating protein 21, ARHGAP21

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

Q5T5U3, A2RUV4, Q6DFG0, Q6DFV3, Q71M21

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Rho GTPase-activating protein 21, Rho GTPase-activating protein 10, Rho-type GTPase-activating protein 21, ARHGAP21

Background

Rho GTPase-activating protein 21 (ARHGAP21) is a ~217 kDa protein. Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions.

Note

For research use only.

Images

References

Datasheet

Anti-ARHGAP21 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ARHGAP21 Polyclonal Antibody (HA562014)

Overview

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Datasheet

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