Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (Nano-7) (VK441063)

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Datasheet

Overview

Catalog No.

VK441063

Description

Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (Nano-7) [Nano-7] (VK441063) is a human monoclonal antibody detecting Capsid protein VP1 in ELISA.

Species reactivity

Norwalk virus (strain GI/Human/United States/Norwalk/1968)(Hu/NV/NV/1968/US)

Applications

ELISA

Host species

Human

Isotype

VHH-hFc

Clone ID

Nano-7

Clonality

Monoclonal

Target

Capsid protein VP1, CP, p59, Soluble capsid protein, Protein 30k, p30, ORF2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q83884

RRID

Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (Nano-7) (abinScience Cat# VK441063, RRID:AB_3729210)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Capsid protein VP1 is a ~56 kDa protein. Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulates the genomic RNA and is decorated with VP2 proteins. Attaches virion to target cells by binding histo-blood group antigens (HBGAs) present on gastroduodenal epithelial cells.

1. Prasad, BV. et al. (1999) Science (New York, N.Y.) 286, 287-90. PMID: 10514371

2. Jung, J. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 12828-12832. PMID: 31182604

3. White, LJ. et al. (1997) Journal of virology 71, 8066-72. PMID: 9311906

4. Marionneau, S. et al. (2002) Gastroenterology 122, 1967-77. PMID: 12055602

5. Huang, P. et al. (2003) The Journal of infectious diseases 188, 19-31. PMID: 12825167

6. Tan, M. et al. (2006) Journal of virology 80, 7322-31. PMID: 16840313

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (Nano-7).
Bioactivity

Detects recombinant Norovirus VP1/p30 (Catalog No: VK441052) in indirect ELISA.

References

Datasheet

Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (Nano-7).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Norovirus/Norwalk virus Capsid protein VP1/p59 Antibody (Nano-7) (VK441063)

Overview

Images

References

Datasheet

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