Anti-DENV-1 Envelope protein E Antibody (1F4) (VK589083)

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1mg

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Datasheet

Overview

Catalog No.

VK589083

Description

Anti-DENV-1 Envelope protein E Antibody (1F4) [1F4] (VK589083) is a human monoclonal antibody detecting Envelope protein E in ELISA. Suitable for Dengue virus 1.

Species reactivity

Dengue virus type 1 (strain Nauru/West Pac/1974) (DENV-1), Dengue virus 1

Applications

ELISA

Host species

Human

Isotype

IgG1, kappa

Clone ID

1F4

Clonality

Monoclonal

Target

Genome polyprotein, Envelope protein E

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P17763, G3F5K5

RRID

Anti-DENV-1 Envelope protein E Antibody (1F4) (abinScience Cat# VK589083, RRID:AB_3729124)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Genome polyprotein (Envelope protein E) is a ~378 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

1. Kuhn, RJ. et al. (2002) Cell 108, 717-25. PMID: 11893341

2. Sangiambut, S. et al. (2008) The Journal of general virology 89, 1254-1264. PMID: 18420804

3. Colpitts, TM. et al. (2011) PloS one 6, e24365. PMID: 21909430

4. Bhuvanakantham, R. et al. (2013) Cellular microbiology 15, 1688-706. PMID: 23522008

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-DENV-1 Envelope protein E Antibody (1F4)

References

Datasheet

Anti-DENV-1 Envelope protein E Antibody (1F4).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DENV-1 Envelope protein E Antibody (1F4) (VK589083)

Overview

Images

References

Datasheet

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