Anti-Neisseria meningitidis FHBP Antibody (1A12) (JN824013)

Datasheet Share product Copy info

Price(USD): $

Spec:

20ug
50ug
100ug
1mg

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

JN824013

Description

Anti-Neisseria meningitidis FHBP Antibody (1A12) [1A12] (JN824013) is a mouse monoclonal antibody detecting fHbp in ELISA.

Species reactivity

Neisseria meningitidis serogroup B (strain MC58)

Applications

ELISA

Host species

Mouse

Isotype

IgG2a

Clone ID

1A12

Clonality

Monoclonal

Target

Factor H binding protein, fHbp, Genome-derived Neisseria antigen 1870, GNA1870, Lipoprotein 2086, LP2086, fhbP, NMB1870

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9JXV4

RRID

Anti-Neisseria meningitidis FHBP Antibody (1A12) (abinScience Cat# JN824013, RRID:AB_3729061)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Factor H binding protein (FHBP) is a ~28 kDa protein. A bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH), binding contributes to the avoidance of complement-mediated lysis by N.meningitidis. Binding of fH to the bacteria surface is independent of bacterial sialic acid moieties. fH binding affinity is high enough that it may sequester plasma fH, depleting its circulating levels and de-regulating complement in the host (Probable). This protein induces high levels of bactericidal antibodies in mice.

1. Schneider, MC. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 7566-75. PMID: 16751403

2. Masignani, V. et al. (2003) The Journal of experimental medicine 197, 789-99. PMID: 12642606

3. Fletcher, LD. et al. (2004) Infection and immunity 72, 2088-100. PMID: 15039331

4. Giuliani, MM. et al. (2005) Infection and immunity 73, 1151-60. PMID: 15664958

5. Scarselli, M. et al. (2011) Science translational medicine 3, 91ra62. PMID: 21753121

6. Johnson, S. et al. (2012) PLoS pathogens 8, e1002981. PMID: 23133374

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Neisseria meningitidis FHBP Antibody (1A12)

References

Datasheet

Anti-Neisseria meningitidis FHBP Antibody (1A12).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Neisseria meningitidis FHBP Antibody (1A12) (JN824013)

Overview

Images

References

Datasheet

Quick access

Contact Us