Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (W1) (JN898053)

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100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.

JN898053

Description

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (W1) [W1] (JN898053) is a mouse monoclonal antibody detecting Bacillus anthracis in ELISA, IP, SPR. Suitable for Bacillus anthracis.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Bacillus anthracis

Applications

ELISA, IP, SPR

Host species

Mouse

Isotype

IgG2a

Clone ID

Clonality

Monoclonal

Target

pagA, PA, Protective antigen, Anthrax toxins translocating protein, PA-83, PA83, Bacillus anthracis

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13423

RRID

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (W1) (abinScience Cat# JN898053, RRID:AB_3729000)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protective antigen (PA/pagA) is a ~85 kDa protein. Protective antigen constitutes one of the three proteins composing the anthrax toxin; it mediates attachment to host cells and translocation of edema factor (EF) and lethal factor (LF) into the host cytoplasm. PA associated with LF forms the lethal toxin (LeTx) and causes death when injected; PA associated with EF forms the edema toxin (EdTx) and produces edema. PA induces immunity to infection with anthrax.

1. Bradley, KA. et al. (2001) Nature 414, 225-9. PMID: 11700562

2. Bradley, KA. et al. (2003) The Journal of biological chemistry 278, 49342-7. PMID: 14507921

3. Santelli, E. et al. (2004) Nature 430, 905-8. PMID: 15243628

4. Lacy, DB. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 13147-51. PMID: 15326297

5. Singh, Y. et al. (1991) The Journal of biological chemistry 266, 15493-7. PMID: 1651334

6. Mock, M. et al. (2001) Annual review of microbiology 55, 647-71. PMID: 11544370

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (W1)

References

Datasheet

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (W1).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (W1) (JN898053)

Overview

Images

References

Datasheet

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