Anti-PRRSV RNA-directed RNA polymerase/NSP9 Nanobody (Nb8) (VK534013)

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1mg

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Datasheet

Overview

Catalog No.

VK534013

Description

Anti-PRRSV RNA-directed RNA polymerase/NSP9 Nanobody (Nb8) [Nb8] (VK534013) is a alpaca monoclonal antibody detecting RNA-directed RNA polymerase in ELISA.

Species reactivity

Porcine reproductive and respiratory syndrome virus (PRRSV), Porcine reproductive and respiratory syndrome virus (strain HB-1) (PRRSV)

Applications

ELISA

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

Nb8

Clonality

Monoclonal

Target

Replicase polyprotein 1ab, ORF1ab polyprotein, RNA-directed RNA polymerase, NSP9

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

A6YQT5, Q8B912

RRID

Anti-PRRSV RNA-directed RNA polymerase/NSP9 Nanobody (Nb8) (abinScience Cat# VK534013, RRID:AB_3727899)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Replicase polyprotein 1ab (RNA-directed RNA polymerase/NSP9) is a ~430 kDa protein. Contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-PRRSV RNA-directed RNA polymerase/NSP9 Nanobody (Nb8)

References

Datasheet

Anti-PRRSV RNA-directed RNA polymerase/NSP9 Nanobody (Nb8).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PRRSV RNA-directed RNA polymerase/NSP9 Nanobody (Nb8) (VK534013)

Overview

Images

References

Datasheet

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