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Anti-Mouse VEGFA Antibody (G6) (MB941013)

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  • Datasheet
Overview
Catalog No.MB941013
Species reactivityHuman, Mouse, Rat, Rabbit
ApplicationsBlocking, ELISA, FCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDG6
Clonality Monoclonal
Target Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q00731
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Bioactivity

    Detects Mouse VEGFA in indirect ELISAs.

  • SDS-PAGE

    SDS-PAGE for Anti-Mouse VEGFA Antibody (G6).

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: MB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
    Lane 2: negative control

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