Anti-Thermus thermophilus SecY Antibody (SAA1468) (JN047013)

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100ug
1mg

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Datasheet

Overview

Catalog No.

JN047013

Description

Anti-Thermus thermophilus SecY Antibody (SAA1468) [SAA1468] (JN047013) is a mouse monoclonal antibody detecting Protein translocase subunit SecY in ELISA.

Species reactivity

Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Applications

ELISA

Host species

Mouse

Isotype

IgG2a

Clone ID

SAA1468

Clonality

Monoclonal

Target

Protein translocase subunit SecY, Sec61α

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q5SHQ8

RRID

Anti-Thermus thermophilus SecY Antibody (SAA1468) (abinScience Cat# JN047013, RRID:AB_3727105)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protein translocase subunit SecY is a ~48 kDa protein. The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Thermus thermophilus SecY Antibody (SAA1468)

References

Datasheet

Anti-Thermus thermophilus SecY Antibody (SAA1468).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Thermus thermophilus SecY Antibody (SAA1468) (JN047013)

Overview

Images

References

Datasheet

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