Anti-Staphylococcus aureus isdA/isdB Antibody (SAA1457) (JN065013)

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Overview

Catalog No.

JN065013

Description

Anti-Staphylococcus aureus isdA/isdB Antibody (SAA1457) [SAA1457] (JN065013) is a mouse monoclonal antibody detecting Staphylococcus aureus surface protein J in ELISA.

Species reactivity

Staphylococcus aureus (strain NCTC 8325 / PS 47) (Q2FZE9), Staphylococcus aureus (strain NCTC 8325 / PS 47) (Q2FZF0)

Applications

ELISA

Host species

Mouse

Isotype

IgG2a

Clone ID

SAA1457

Clonality

Monoclonal

Target

isdA, frpA, stbA, Iron-regulated surface determinant protein A, Fur-regulated protein A, Staphylococcal transferrin-binding protein A, isdB, frpB, sasJ, sirH, Iron-regulated surface determinant protein B, Fur-regulated protein B, Staphylococcal iron-regulated protein H, Staphylococcus aureus surface protein J

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q2FZE9, Q2FZF0

RRID

Anti-Staphylococcus aureus isdA/isdB Antibody (SAA1457) (abinScience Cat# JN065013, RRID:AB_3727086)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Iron-regulated surface determinant protein A (isdA/isdB) is a ~38 kDa protein. Cell wall-anchored surface receptor that participates in the extraction of heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source. Receives heme from IsdB and transfers it to IsdC. Also plays a role in the inhibition of host immune response. Protects S.aureus against the bactericidal protease activity of apolactoferrin. Decreases bacterial cellular hydrophobicity, which renders S.aureus resistant to bactericidal human skin fatty acids as well as to beta-defensins and cathelicidin.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Staphylococcus aureus isdA/isdB Antibody (SAA1457)

References

Datasheet

Anti-Staphylococcus aureus isdA/isdB Antibody (SAA1457).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Staphylococcus aureus isdA/isdB Antibody (SAA1457) (JN065013)

Overview

Images

References

Datasheet

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