Anti-Clostridium botulinum botA/BOTOX Nanobody (SAA0934) (JN888143)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

JN888143

Description

Anti-Clostridium botulinum botA/BOTOX Nanobody (SAA0934) [SAA0934] (JN888143) is a alpaca monoclonal antibody detecting BOTOX in ELISA.

Species reactivity

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Applications

ELISA

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA0934

Clonality

Monoclonal

Target

Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin A light chain, LC, 3.4.24.69, Botulinum neurotoxin A heavy chain, HC, botA, bna, CBO0806, CLC_0862

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P0DPI1

RRID

Anti-Clostridium botulinum botA/BOTOX Nanobody (SAA0934) (abinScience Cat# JN888143, RRID:AB_3726843)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Botulinum neurotoxin type A (botA/BOTOX) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Blasi, J. et al. (1993) Nature 365, 160-3. PMID: 8103915

2. Fu, Z. et al. (2009) Biochemistry 48, 5631-41. PMID: 19476346

Note

For research use only.

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