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Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380) (VK425043)

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Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380)

Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380)

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Datasheet

Overview

Catalog No.	VK425043
Description	Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380) [DC1.380] (VK425043) is a human monoclonal antibody detecting Structural polyprotein in FCM, IP. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Chikungunya virus (strain S27-African prototype) (CHIKV)
Applications	FCM, IP
Host species	Human
Isotype	IgG1, kappa
Clone ID	DC1.380
Clonality	Monoclonal
Target	p130, Structural polyprotein
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q8JUX5
RRID	Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380) (abinScience Cat# VK425043, RRID:AB_3725330)
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Structural polyprotein (Spike glycoprotein E1) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Webb, LG. et al. (2020) PLoS pathogens 16, e1008999. PMID: 33057424
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380).

References

Datasheet

Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380).pdf

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Anti-CHIKV p130/Structural polyprotein Antibody (DC1.380)