Anti-CHPV N/Nucleoprotein Polyclonal Antibody (VK773014)

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50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

VK773014

Description

Anti-CHPV N/Nucleoprotein Polyclonal Antibody (VK773014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Chandipura virus (strain I653514) (CHPV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Chandipura virus (CHPV) N/Nucleoprotein (Met1-Ala422).

Target

Nucleoprotein, NP, Nucleocapsid protein, Protein N, N

Purification

Purified by antigen affinity column.

Accession

P11211

RRID

Anti-CHPV N/Nucleoprotein Polyclonal Antibody (abinScience Cat# VK773014, RRID:AB_3725150)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-N-CHPV polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant CHPV N/Nucleoprotein (Catalog No: VK773012)

References

Datasheet

Anti-CHPV N/Nucleoprotein Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CHPV N/Nucleoprotein Polyclonal Antibody (VK773014)

Overview

Images

References

Datasheet

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