Anti-Staphylococcus aureus entC2/SEC2 Polyclonal Antibody (JN880014)

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100ug

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Overview
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Datasheet

Overview

Catalog No.

JN880014

Description

Anti-Staphylococcus aureus entC2/SEC2 Polyclonal Antibody (JN880014) is a rabbit polyclonal antibody detecting entC2 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Staphylococcus aureus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Staphylococcus aureus entC2/SEC2 (Glu28-Gly266).

Target

Enterotoxin type C-2, SEC2, entC2

Purification

Purified by antigen affinity column.

Accession

P34071

RRID

Anti-Staphylococcus aureus entC2/SEC2 Polyclonal Antibody (abinScience Cat# JN880014, RRID:AB_3725082)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Enterotoxin type C-2 (entC2/SEC2) is a ~30 kDa protein. Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines. Also causes the intoxication staphylococcal food poisoning syndrome.

1. Papageorgiou, AC. et al. (2004) The Journal of biological chemistry 279, 1297-303. PMID: 14559915

2. Wang, X. et al. (2009) Microbiology (Reading, England) 155, 680-686. PMID: 19246739

3. Dohlsten, M. et al. (1990) Immunology 71, 96-100. PMID: 2210803

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Staphylococcus aureus entC2/SEC2 polyclonal antibody at 1ug/mL on recombinant Staphylococcus aureus entC2/SEC2 (Catalog No. JN880012).

References

Datasheet

Anti-Staphylococcus aureus entC2/SEC2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Staphylococcus aureus entC2/SEC2 Polyclonal Antibody (JN880014)

Overview

Images

References

Datasheet

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