Anti-Human ULK1 Polyclonal Antibody (HF942014)

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Datasheet

Overview

Catalog No.

HF942014

Description

Anti-Human ULK1 Polyclonal Antibody (HF942014) is a rabbit polyclonal antibody detecting ULK1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ULK1 (Met1-His275).

Target

ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, KIAA0722, Serine/threonine-protein kinase ULK1, hATG1, ATG1

Purification

Purified by antigen affinity column.

Accession

O75385

RRID

Anti-Human ULK1 Polyclonal Antibody (abinScience Cat# HF942014, RRID:AB_3725069)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase ULK1 is a ~112 kDa protein. Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences.

1. Chan, EY. et al. (2009) Molecular and cellular biology 29, 157-71. PMID: 18936157

2. Löffler, AS. et al. (2011) Autophagy 7, 696-706. PMID: 21460634

3. Jung, CH. et al. (2011) Autophagy 7, 1212-21. PMID: 21795849

4. Nazio, F. et al. (2013) Nature cell biology 15, 406-16. PMID: 23524951

5. Ro, SH. et al. (2014) The FEBS journal 281, 3816-27. PMID: 25040165

6. Kim, JH. et al. (2018) EMBO reports 19. PMID: 29487085

7. Di Rienzo, M. et al. (2019) Science advances 5, eaau8857. PMID: 31123703

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ULK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate

References

Datasheet

Anti-Human ULK1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ULK1 Polyclonal Antibody (HF942014)

Overview

Images

References

Datasheet

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