Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014)

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Overview
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Datasheet

Overview

Catalog No.

JN971014

Description

Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014) is a rabbit polyclonal antibody detecting autotransporter adhesin NadA in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Neisseria meningitidis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Neisseria meningitidis NadA (Ala39-Ala170).

Target

autotransporter adhesin NadA, partial, autotransporter adhesin NadA, partial

Purification

Purified by antigen affinity column.

Accession

P0DV44

RRID

Anti-Neisseria meningitidis NadA Polyclonal Antibody (abinScience Cat# JN971014, RRID:AB_3724896)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Neisseria adhesin A (NadA) is a ~42 kDa protein. Adheres to and induces bacterial uptake by human epithelial cells in a microfilament-dependent process. Binding is reduced by pronase treatment, suggesting there is a protein receptor on the human cells. Possible human protein receptors include integrin beta-1 (ITGB1) and oxidized low-density lipoprotein receptor 1 (OLR1) (Probable). Binds to extracellular human Hsp90 (preferentially the beta isoform, HSP90AB1) on monocytes, binding stimulates monocytes in a TLR4-dependent fashion, polymixin B, which binds NadA, blocks the activation. Hsp90 is probably not the first receptor on human monocytes.

1. Capecchi, B. et al. (2005) Molecular microbiology 55, 687-98. PMID: 15660996

2. Liguori, A. et al. (2018) mBio 9. PMID: 30327444

3. Cecchini, P. et al. (2011) PloS one 6, e25089. PMID: 21949862

4. Bozza, G. et al. (2014) PloS one 9, e110047. PMID: 25347845

5. Comanducci, M. et al. (2002) The Journal of experimental medicine 195, 1445-54. PMID: 12045242

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Neisseria meningitidis NadA polyclonal antibody at 1ug/mL on recombinant Neisseria meningitidis NadA (Catalog No. JN971012).

References

Datasheet

Anti-Neisseria meningitidis NadA Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014)

Overview

Images

References

Datasheet

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