Anti-FUS Polyclonal Antibody (HW826014)

Datasheet Share product Copy info

Price(USD): $

Spec:

20ug
50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HW826014

Description

Anti-FUS Polyclonal Antibody (HW826014) is a rabbit polyclonal antibody detecting FUS in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FUS (Ser282-Ser367).

Target

RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein, FUS, TLS

Purification

Purified by antigen affinity column.

Accession

P35637

RRID

Anti-FUS Polyclonal Antibody (abinScience Cat# HW826014, RRID:AB_3724170)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RNA-binding protein FUS is a ~53 kDa protein. DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Also binds its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair.

1. Yamaguchi, A. et al. (2016) Scientific reports 6, 35195. PMID: 27731383

2. Nguyen, CD. et al. (2011) Journal of molecular biology 407, 273-83. PMID: 21256132

3. Yu, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8608-13. PMID: 26124092

4. Zhou, Y. et al. (2013) PLoS genetics 9, e1003895. PMID: 24204307

5. Baechtold, H. et al. (1999) The Journal of biological chemistry 274, 34337-42. PMID: 10567410

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FUS polyclonal antibody at 1ug/mL on various samples.
Lane 1: N2A cell lysate
Lane 2: HepG2 cell lysate