Anti-TP53/p53 Polyclonal Antibody (HY039024)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HY039024

Description

Anti-TP53/p53 Polyclonal Antibody (HY039024) is a rabbit polyclonal antibody detecting TP53 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TP53/p53 (Ser94-Thr312).

Target

Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53

Purification

Purified by antigen affinity column.

Accession

P04637

RRID

Anti-TP53/p53 Polyclonal Antibody (abinScience Cat# HY039024, RRID:AB_3724085)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cellular tumor antigen p53 (TP53) is a ~43 kDa protein. Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

1. Guo, A. et al. (2000) Nature cell biology 2, 730-6. PMID: 11025664

2. Bergamaschi, D. et al. (2003) Nature genetics 33, 162-7. PMID: 12524540

3. Louria-Hayon, I. et al. (2003) The Journal of biological chemistry 278, 33134-41. PMID: 12810724

4. An, W. et al. (2004) Cell 117, 735-48. PMID: 15186775

5. Ghosh, A. et al. (2004) Molecular and cellular biology 24, 7987-97. PMID: 15340061

6. Zhao, Y. et al. (2007) The Journal of biological chemistry 282, 11969-81. PMID: 17317671

7. Taira, N. et al. (2007) Molecular cell 25, 725-38. PMID: 17349958

8. Allton, K. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 11612-6. PMID: 19556538

9. Huarte, M. et al. (2010) Cell 142, 409-19. PMID: 20673990

10. Wu, L. et al. (2011) The Journal of biological chemistry 286, 2236-44. PMID: 20959462

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TP53/p53 polyclonal antibody at 1ug/mL on recombinant TP53/p53 (Catalog No. HY039032).

References

Datasheet

Anti-TP53/p53 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TP53/p53 Polyclonal Antibody (HY039024)

Overview

Images

References

Datasheet

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