Anti-VCP Polyclonal Antibody (HX922014)

Name: Anti-VCP Polyclonal Antibody
Brand: abinScience
SKU: HX922014
Price: 96 USD
Availability: InStock

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Spec:

20ug
50ug
100ug

Number:

Overview
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References
Datasheet

Overview

Catalog No.

HX922014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human VCP (Gly125-Ile371).

Target

Transitional endoplasmic reticulum ATPase, Valosin-containing protein, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, VCP

Purification

Purified by antigen affinity column.

Accession

P55072

RRID

Anti-VCP Polyclonal Antibody (abinScience Cat# HX922014, RRID:AB_3723235)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transitional endoplasmic reticulum ATPase (VCP) is a ~89 kDa protein. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome.

1. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139

2. Kadowaki, H. et al. (2015) Cell reports 13, 944-56. PMID: 26565908

3. Turakhiya, A. et al. (2018) Molecular cell 70, 906-919.e7. PMID: 29804830

4. Gwon, Y. et al. (2021) Science (New York, N.Y.) 372, eabf6548. PMID: 34739333

5. Meerang, M. et al. (2011) Nature cell biology 13, 1376-82. PMID: 22020440

6. Acs, K. et al. (2011) Nature structural & molecular biology 18, 1345-50. PMID: 22120668

7. Mosbech, A. et al. (2012) Nature structural & molecular biology 19, 1084-92. PMID: 23042605

8. Davis, EJ. et al. (2012) Nature structural & molecular biology 19, 1093-100. PMID: 23042607

9. Fielden, J. et al. (2020) Nature communications 11, 1274. PMID: 32152270

10. Krastev, DB. et al. (2022) Nature cell biology 24, 62-73. PMID: 35013556

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-VCP polyclonal antibody at 1ug/mL on various samples.
Lane 1: U251 cell lysate
Lane 2: Mouse lung lysate
Lane 3: Rat brain lysate

References

Datasheet

Anti-VCP Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-VCP Polyclonal Antibody (HX922014)

Overview

Images

References

Datasheet

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