Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody (JN886014)

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100ug

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Overview
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Datasheet

Overview

Catalog No.

JN886014

Description

Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody (JN886014) is a rabbit polyclonal antibody detecting Streptopain in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Streptococcus pyogenes, Streptococcus pyogenes serotype M1, Streptococcus pyogenes serotype M18, Streptococcus pyogenes serotype M28, Streptococcus pyogenes serotype M3, Streptococcus pyogenes serotype M6

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Streptococcus pyogenes speB/Streptopain (Gln146-Pro398).

Target

Streptopain, 3.4.22.10, Exotoxin type B, SPE B, Streptococcal cysteine proteinase, Streptococcus peptidase A, SPP, speB

Purification

Purified by antigen affinity column.

Accession

P0C0J0

RRID

Anti-Streptococcus pyogenes speB/Streptopain Polyclonal Antibody (abinScience Cat# JN886014, RRID:AB_3730592)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Streptopain (speB) is a ~43 kDa protein. Cysteine protease that acts as a key streptococcal virulence factor by cleaving host proteins involved in immune response. Triggers inflammation by mediating cleavage of host proteins, which can both promote host pathogenesis by triggering sterile inflammation and/or restrict streptococcal infection, depending on host immune statue and infection site. Cleaves host gasdermin-A (GSDMA) in epithelial cells, promoting GSDMA activation and formation of gasdermin pores, triggering pyroptosis. Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response. This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen.

1. Doran, JD. et al. (1999) European journal of biochemistry 263, 145-51. PMID: 10429198

2. Nomizu, M. et al. (2001) The Journal of biological chemistry 276, 44551-6. PMID: 11553627

3. Yu, CE. et al. (1991) Infection and immunity 59, 211-5. PMID: 1987034

4. González-Páez, GE. et al. (2012) The Journal of biological chemistry 287, 24412-26. PMID: 22645124

5. Musser, JM. et al. (1996) Infection and immunity 64, 1913-7. PMID: 8675287

6. Lukomski, S. et al. (1997) The Journal of clinical investigation 99, 2574-80. PMID: 9169486

7. Kuo, CF. et al. (1999) Infection and immunity 67, 126-30. PMID: 9864206

8. Kapur, V. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 7676-80. PMID: 7689226

9. Kapur, V. et al. (1993) Microbial pathogenesis 15, 327-46. PMID: 7516997

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Streptococcus pyogenes speB/Streptopain polyclonal antibody at 1ug/mL on recombinant Streptococcus pyogenes speB/Streptopain (Catalog No. JN886012).

References

Datasheet