Anti-TBK1 Polyclonal Antibody (HV178014)

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50ug
100ug

Number:

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Datasheet

Overview

Catalog No.

HV178014

Description

Anti-TBK1 Polyclonal Antibody (HV178014) is a rabbit polyclonal antibody detecting TBK1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TBK1 (Met1-Gly121).

Target

Serine/threonine-protein kinase TBK1, NAK, TANK-binding kinase 1, T2K, TBK1, NF-kappa-B-activating kinase

Purification

Purified by antigen affinity column.

Accession

Q9UHD2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase TBK1 is a ~83 kDa protein. Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, STING1 and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1.

1. Pomerantz, JL. et al. (1999) The EMBO journal 18, 6694-704. PMID: 10581243

2. Kishore, N. et al. (2002) The Journal of biological chemistry 277, 13840-7. PMID: 11839743

3. Fitzgerald, KA. et al. (2003) Nature immunology 4, 491-6. PMID: 12692549

4. Sharma, S. et al. (2003) Science (New York, N.Y.) 300, 1148-51. PMID: 12702806

5. Mori, M. et al. (2004) The Journal of biological chemistry 279, 9698-702. PMID: 14703513

6. tenOever, BR. et al. (2004) Journal of virology 78, 10636-49. PMID: 15367631

7. Kuai, J. et al. (2004) The Journal of biological chemistry 279, 53266-71. PMID: 15485837

8. Soulat, D. et al. (2008) The EMBO journal 27, 2135-46. PMID: 18583960

9. Clark, K. et al. (2011) The Biochemical journal 434, 93-104. PMID: 21138416

10. Larabi, A. et al. (2013) Cell reports 3, 734-46. PMID: 23453971

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TBK1 polyclonal antibody at 1ug/mL on recombinant TBK1 (Catalog No. HV178012).

References

Datasheet

Anti-TBK1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TBK1 Polyclonal Antibody (HV178014)

Overview

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References

Datasheet

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