Anti-TrkB /NTRK2 Polyclonal Antibody (HS892014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HS892014

Description

Anti-TrkB /NTRK2 Polyclonal Antibody (HS892014) is a rabbit polyclonal antibody detecting NTRK2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TrkB /NTRK2 (Cys32-His430).

Target

Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB, TRKB, NTRK2, Tropomyosin-related kinase B, BDNF/NT-3 growth factors receptor, Trk-B

Purification

Purified by antigen affinity column.

Accession

Q16620

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

BDNF/NT-3 growth factors receptor (TrkB/NTRK2) is a ~91 kDa protein. Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades.

1. Yeo, GS. et al. (2004) Nature neuroscience 7, 1187-9. PMID: 15494731

2. Haniu, M. et al. (1995) Archives of biochemistry and biophysics 322, 256-64. PMID: 7574684

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TrkB /NTRK2 polyclonal antibody at 1ug/mL on recombinant TrkB /NTRK2 (Catalog No. HS892012).

References

Datasheet