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InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43) (MF815109)

InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43)
InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43)
InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43)
InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43)
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Overview
Catalog No.MF815109
Description
InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43) [2.43] (MF815109) is a rat monoclonal antibody detecting T-cell surface glycoprotein CD8 alpha chain in FCM, in vivo CD8+ T cell depletion, Western blot, Neutralization. Suitable for Mouse.
Highlights
  • Multi-Application — Validated across multiple applications.
  • Flow Cytometry — Validated for flow cytometry applications.
Species reactivityMouse
ApplicationsFCM, in vivo CD8+ T cell depletion, Western blot, Neutralization
Host speciesRat
IsotypelgG2b
Clone ID2.43
Clonality Monoclonal
Target T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2, cd8
Endotoxin level <1 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P01731
RRID InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43) (abinScience Cat# MF815109, RRID:AB_3730135)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~27 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

Note For research use only.
Images
  • InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43)

    SDS-PAGE

    SDS-PAGE for InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43)

  • InVivo Plus Anti-Mouse CD8a/Lyt2 Antibody (2.43)

    Bioactivity

    Detects CD8a/Lyt2 (Catalog No. MF815011) in indirect ELISAs.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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