Affiliations
Caroline L Holley, Mercedes Monteleone, Daniel Fisch, Alexandre E S Libert, Robert J Ju, Joon H Choi, Nicholas D Condon, Stefan Emming, Joanna Crawford, Grace M E P Lawrence, Jared R Coombs, James G Lefevre, Rinie Bajracharya, Mireille H Lahoud, Alpha S Yap, Nicholas Hamilton, Samantha J Stehbens, Jonathan C Kagan, Nicholas Ariotti, Sabrina S Burgener, Kate Schroder
DOI: 10.1038/s41590-024-02024-3
Abstract
While apoptosis dismantles the cell to enforce immunological silence, pyroptotic cell death provokes inflammation. Little is known of the structural architecture of cells undergoing pyroptosis, and whether pyroptotic corpses are immunogenic. Here we report that inflammasomes trigger the Gasdermin-D- and calcium-dependent eruption of filopodia from the plasma membrane minutes before pyroptotic cell rupture, to crown the resultant corpse with filopodia. As a rich store of F-actin, pyroptotic filopodia are recognized by dendritic cells through the F-actin receptor, CLEC9A (DNGR1). We propose that cells assemble filopodia before cell rupture to serve as a posthumous mark for a cell that has died by gasdermin-induced pyroptosis, or MLKL-induced necroptosis, for recognition by dendritic cells. This study reveals the spectacular morphology of pyroptosis and identifies a mechanism by which inflammasomes induce pyroptotic cells to construct a de novo alarmin that activates dendritic cells via CLEC9A, which coordinates the transition from innate to adaptive immunity
Keywords
DNGR1，CD24，CLEC9A
