Энзимы

Q: What does “carrier-free” mean?

Carrier-free proteins contain no stabilizers (e.g., BSA), reducing interference in sensitive assays and enabling precise mass-based dosing. Ideal for SPR, BLI, and cell-based functional studies.

Q: Which expression system should I choose?

HEK293/CHO: Native folding, glycosylation, and disulfide bonds — ideal for receptor-binding and functional studies.E. coli: High yield and cost-effective, best for structural screening.Insect/Yeast: Balanced PTMs with good scalability.

Q: What endotoxin level is recommended for immune cell assays?

≤0.1 EU/µg is the standard for immune cell culture, T-cell expansion, and in vivo studies. Every lot ships with LAL-verified endotoxin data on the CoA.

Q: How to reconstitute lyophilized proteins?

Use sterile, endotoxin-free buffer. Gently swirl — avoid vortexing. Aliquot into single-use volumes and store at -80°C to prevent freeze-thaw damage.

Q: Are these proteins RUO or GMP-grade?

Unless explicitly labeled, all products are Research Use Only (RUO). For GMP-grade requirements, contact our team for custom manufacturing options.

Почему исследователи выбирают abinScience

Чистота ≥95% и низкий уровень эндотоксина Typical ≤0.1 EU/µg verified by LAL assay. Lot-specific CoA with SDS-PAGE and SEC-HPLC data included.

Подтвержденная биологическая активность Functional validation via ELISA or cell-based proliferation/signaling assay. High batch-to-batch consistency (<20% CV).

Пять экспрессионных систем HEK293 & CHO for native PTMs; E. coli for high-yield screening; insect and yeast for balanced cost and glycosylation.

Гибкие форматы Carrier-free (no BSA), His-tagged, Fc-chimera, biotinylated; lyophilized or liquid — choose the best fit for your assay.

Масштабируемый и надежный From µg to mg quantities with consistent lead times. Global cold-chain shipping with dry-ice packaging.

Как выбрать подходящий продукт

Применение:

Choose carrier-free for sensitive bioassays (e.g., CAR-T expansion, organoid culture, SPR). His-tag works best for pull-down and purification workflows.

Экспрессионная система:

Use HEK293/CHO for native glycosylation and disulfide bonds; E. coli for cost-effective high-throughput screening; insect/yeast when moderate PTMs are sufficient.

Тег и формат:

His-tag for IMAC purification and binding studies; Fc-chimera for enhanced avidity and half-life; biotinylated for immobilization on streptavidin surfaces.

Эндотоксин:

Match your assay tolerance — ≤0.1 EU/µg is recommended for immune cell culture and in vivo studies. Always verify via the lot-specific CoA.

Документы и данные QC

Download Certificate of Analysis (CoA), SDS-PAGE gels, SEC-HPLC chromatograms, and reconstitution protocols on each product page. Все данные контроля качества (QC) специфичны для конкретной партии и архивируются для обеспечения прослеживаемости.

Часто задаваемые вопросы (ЧЗВ)

Что означает «без носителя»?

Carrier-free proteins contain no stabilizers (e.g., BSA), reducing interference in sensitive assays and enabling precise mass-based dosing. Ideal for SPR, BLI, and cell-based functional studies.

Какую систему экспрессии мне выбрать?

HEK293/CHO: Native folding, glycosylation, and disulfide bonds — ideal for receptor-binding and functional studies.
E. coli: High yield and cost-effective, best for structural screening.
Insect/Yeast: Balanced PTMs with good scalability.

Какой уровень эндотоксина рекомендуется для анализов с использованием иммунных клеток?

≤0.1 EU/µg is the standard for immune cell culture, T-cell expansion, and in vivo studies. Every lot ships with LAL-verified endotoxin data on the CoA.

Как восстановить лиофилизированные белки?

Use sterile, endotoxin-free buffer. Gently swirl — avoid vortexing. Aliquot into single-use volumes and store at -80°C to prevent freeze-thaw damage.

Эти белки предназначены для исследовательских целей (RUO) или произведены по стандартам GMP?

Unless explicitly labeled, all products are Research Use Only (RUO). For GMP-grade requirements, contact our team for custom manufacturing optio