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Recombinant JEV Envelope protein E Protein, N-TrxA-His & C-His (VK731012)

Recombinant JEV Envelope protein E Protein, N-TrxA-His & C-His
Recombinant JEV Envelope protein E Protein, N-TrxA-His & C-His
Цена(USD): $
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

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  • Ссылки

  • Даташит

Обзор
Каталожный номерVK731012
Описание
Recombinant JEV Envelope protein E Protein, N-TrxA-His & C-His (VK731012) expressed in E. coli, spanning Phe295-Ala794. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаP32886
Длина белкаPhe295-Ala794
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыJapanese encephalitis virus (strain Jaoars982) (JEV)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 73.48 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияGenome polyprotein, Envelope protein E
Фон

Genome polyprotein (Envelope protein E) is a ~380 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

Примечание For research use only.
Изображения
  • Recombinant JEV Envelope protein E Protein, N-TrxA-His & C-His

    SDS-PAGE

    SDS-PAGE for Recombinant JEV Envelope protein E Protein, N-TrxA-His & C-His

Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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