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Recombinant Human IL5 Protein, N-His (HY218012)

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Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Recombinant Human IL5 Protein, N-His

Цена(USD): $

Спецификация:

50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит

Обзор

Каталожный номер	HY218012
Описание	Recombinant Human IL5 Protein, N-His (HY218012) expressed in E. coli, spanning Ile20-Ser134. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P05113
Длина белка	Ile20-Ser134
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	16.31 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	IL5, B-cell differentiation factor I, IL-5, T-cell replacing factor, TRF, Eosinophil differentiation factor, Interleukin-5
Фон	Interleukin-5 (IL5) is a ~15 kDa protein. Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. IL5 is the therapeutic target of mepolizumab (Nucala). 1. Clutterbuck, EJ. et al. (1989) Blood 73, 1504-12. PMID: 2653458 2. Ochiai, K. et al. (1997) Clinical and experimental immunology 107, 198-204. PMID: 9010276 3. Tavernier, J. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 7041-5. PMID: 1495999 4. Kusano, S. et al. (2012) Protein science : a publication of the Protein Society 21, 850-64. PMID: 22528658 5. Alam, R. et al. (1995) International archives of allergy and immunology 107, 226-7. PMID: 7613138
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human IL5 Protein, N-His
Bioactivity

Detected by Research Grade Depemokimab (Cat No.: HY218036) in indirect ELISA.
Western blot

Detected by Anti-IL5 monoclonal antibody (Catalog No: HF572010)
Bioactivity

Detected by Varokibart (Catalog No: HY218046) in indirect ELISA.

Ссылки

HY218012-SDS-PAGE-1.jpg

Даташит

Recombinant Human IL5 Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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Recombinant Human IL5 Protein, N-His