Anti-Rabies virus/RABV G/Glycoprotein Polyclonal Antibody, Biotin (VK632324)

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概要

カタログ番号

VK632324

説明

Anti-Rabies virus/RABV G/Glycoprotein Polyclonal Antibody, Biotin (VK632324) is a rabbit polyclonal antibody detecting Glycoprotein in WB, IHC, ELISA.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Biotin Conjugated — Signal amplification with streptavidin systems.

種反応性

Rabies virus

アプリケーション

WB, IHC, ELISA

宿主種

Rabbit

アイソタイプ

IgG

抱合

Biotin

クローン性

Polyclonal

免疫原

E. coli - derived recombinant Rabies virus/RABV G/Glycoprotein (Leu30-Tyr459).

ターゲット

G, Glycoprotein

エンドトキシンレベル

Please contact with the lab for this information.

純度

>90% as determined by SDS-PAGE.

精製

Purified by antigen affinity column.

アクセッション番号

O92284

RRID

Anti-Rabies virus/RABV G/Glycoprotein Polyclonal Antibody, Biotin (abinScience Cat# VK632324, RRID:AB_3730118)

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

発送

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glycoprotein is a ~58 kDa protein. Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells.

注意事項

For research use only.

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