Research Grade ombetoxabart (JN876026)

データシート製品共有情報コピー

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100ug
1mg

数量:

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概要
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参考文献
データシート

概要

カタログ番号

JN876026

説明

Research Grade ombetoxabart (JN876026) is a human monoclonal antibody. Suitable for Clostridioides difficile.

種反応性

Clostridioides difficile

アプリケーション

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

宿主種

Human

アイソタイプ

IgG1, kappa

クローン性

Monoclonal

エンドトキシンレベル

< 10 EU/mg

純度

>95% as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

P18177

形態

Liquid

保存バッファー

0.01 M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -101°C long term.

背景

Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149

3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933

5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463

6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

注意事項

For research use only.

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参考文献