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Anti-EV71 VP1/Capsid protein VP1 Antibody (D5) (VK633013)

Anti-EV71 VP1/Capsid protein VP1 Antibody (D5)
Anti-EV71 VP1/Capsid protein VP1 Antibody (D5)
Anti-EV71 VP1/Capsid protein VP1 Antibody (D5)
Anti-EV71 VP1/Capsid protein VP1 Antibody (D5)
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概要
カタログ番号VK633013
説明
Anti-EV71 VP1/Capsid protein VP1 Antibody (D5) [D5] (VK633013) is a human monoclonal antibody detecting Capsid protein VP1 in ELISA, FCM, IF, WB.
Highlights
  • Multi-Application — Validated across multiple applications.
  • Flow Cytometry — Validated for flow cytometry applications.
種反応性Human enterovirus 71 (EV71) (EV-71)
アプリケーションELISA, FCM, IF, WB
宿主種Human
アイソタイプIgG1
クローンIDD5
クローン性 Monoclonal
ターゲット Genome polyprotein, P1, Capsid protein VP1, P1D, Virion protein 1
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 Q5DW45
RRID

Anti-EV71 VP1/Capsid protein VP1 Antibody (D5) (abinScience Cat# VK633013, RRID:AB_3727937)

形態 Liquid
保存バッファー 0.01M PBS, pH 7.4.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:2000-1:20000
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Genome polyprotein (Capsid protein VP1) is a ~242 kDa protein. Acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. During genome replication, the VPg-RNA linkage is removed by the host TDP2, thereby accelerating replication.

注意事項 For research use only.
イメージ
  • Anti-EV71 VP1/Capsid protein VP1 Antibody (D5)

    SDS-PAGE

    SDS-PAGE for Anti-EV71 VP1/Capsid protein VP1 Antibody (D5).

  • Anti-EV71 VP1/Capsid protein VP1 Antibody (D5)

    Bioactivity

    Detects Recombinant EV71 VP1/Capsid protein VP1 Protein, N-His (Cat No.: VK633012) in indirect ELISA.

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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