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Research Grade Abituzumab
Overview
Catalog No. HY065016
Host species Humanized
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target MSK8, VNRA, Vitronectin receptor, Integrin alpha-V, Vitronectin receptor subunit alpha, CD51, ITGAV, VTNR
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact the lab for this information.
Expression system Mammalian Cells
Accession P06756
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative nameDI-17E6, EMD525797, 1105038-73-0
Note For research use only. Not suitable for clinical or therapeutic use.
Images


Bioactivity		 SEC-HPLC detection for Research Grade Abituzumab.

Flow Cytometry		 Flow-cytometry using anti-human CD51 antibody. A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD51 monoclonal antibody (Catalog HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for Research Grade Abituzumab.

Flow CytoMetry		 Flow-cytometry using APC anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using PE anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.