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Anti-Human CD112/Nectin-2/PVRL2 Antibody (SAA0405)
Overview
Catalog No.
HV456107
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.09% Sodium azide.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
ELISA, FCM
Target
Herpesvirus entry mediator B, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, HVEB, PVRL2 receptor-related protein 2, CD112, PRR2, Nectin-2, HveB, NECTIN2
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
Q92692
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SAA0405
Note
For research use only.
Images

Bioactivity

Detects CD112/Nectin-2/PVRL2 in indirect ELISAs.

Flow Cytometry

Flow-cytometry using anti-human Nectin2 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog # HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry

Flow-cytometry using anti-human Nectin2 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Nectin2 antibody monoclonal antibody (Catalog # HV456107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry

Flow-cytometry using anti-human CD112 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD112 monoclonal antibody (Catalog: HV456107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.