Anti-Human CD47/MER6 Antibody (B6H12)

Catalog No.	HC359207
Host species	Mouse
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1, kappa
Applications	FCM
Target	Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	Q08722
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	B6H12
Note	For research use only.

SEC-HPLC	SEC-HPLC detection for Anti-Human CD47/MER6 Antibody (B6H12).
Bioactivity	Detects CD47/MER6 in indirect ELISAs.
Flow Cytometry	Flow-cytometry using anti-human CD47 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human CD47 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human CD47 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human CD47 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human CD47 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD47 monoclonal antibody (Catalog: HC359207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.