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Anti-Human CD28 Antibody (15E8#)
Overview
Catalog No. HY422207
Host species Mouse
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1
Applications FCM
Target CD28, TP44, T-cell-specific surface glycoprotein CD28
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P10747
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID15E8#
Note For research use only.
Images


Bioactivity
SEC-HPLC detection for Anti-Human CD28 Antibody (15E8#).

Flow CytoMetry
Flow-cytometry using anti-human CD28 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD28 monoclonal antibody (Catalog: HY422207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.