| Catalog No. | HW310053 |
| Host species | Rat |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | ELISA, FCM, WB |
| Target | GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P51654 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | YP7 |
| Note | For research use only. |
Rat Anti-Human GPC3 Antibody (YP7)
Overview
Images
 Bioactivity |
Detects recombinant human Glypican-3/GPC3 (Cat No.: HW310011) in indirect ELISA. |
 Bioactivity |
Detects recombinant mouse Glypican-3/GPC3 (Cat No.: MW310011) in indirect ELISA. |
 SDS-PAGE |
SDS-PAGE for Rat Anti-Human GPC3 Antibody (YP7). |
 Flow CytoMetry |
Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310053) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (abinScience: ？) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human GPC3 (Catalog No: HW310011) Lane 2: negative control |