| Catalog No. | HV974063 |
| Host species | Human |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | VHH-hFc |
| Applications | ELISA, WB |
| Target | B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | Q9NZQ7 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | SAA2018 |
| Note | For research use only. |
Anti-Human CD274/PD-L1/B7-H1 Antibody (SAA2018)
Overview
Images
 Bioactivity |
Detects Human CD274/PD-L1/B7-H1 in indirect ELISAs. |
 Flow CytoMetry |
Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: HV974063) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-CD274/PD-L1/B7-H1 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 50ng Lane 2: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 30ng Lane 3: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 20ng Lane 4: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 10ng |